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KMID : 1147820210100020108
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Journal of Naturopathy
2021 Volume.10 No. 2 p.108 ~ p.113
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HPLC Fractionation of Antioxidant Substances of E. hyemale Extract and Analysis of Indicator Components Using LC-MS
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Song Jin-Hwa
Lee Geo-Lyong
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Abstract
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Background: Results on the action of antioxidants in extracts of Equisetum hyemale stems and roots have already been reported, but the antioxidant properties have not been analyzed.
Purpose: This study was to determine the molecular structure of antioxidants in substances extracted from E. hyemale stems and roots.
Methods: Component analysis was analyzed by HPLC and LC-MS after extraction with hot water and ethyl alcohol.
Results: The HPLC chromatogram of stem and root extracts showed four significant peaks at a wavelength of 205 nm. Peak 1 at 280 nm is a typical simple phenolic type, and both peaks 2 - 4 near 280 nm and 370 nm are typical flavonoid glycosides. As for the antioxidant level of the extract by HPLC analysis, the sum of the peaks at 740 nm was the highest at 3,669 mAU in the 100% ethanol extract, 3,096 mAU in the 70% ethanol extract, and 2,868 mAU in the hot water extract. As a result of LC-MS analysis of the antioxidant extract, kaempferol-3-sophoroside-7-glucoside with a molecular weight of 772 da at peak 3, and kaempferol-3-sophoroside-8-glucoside with a molecular weight of 788 and 772 at peak 4 was identified.
Conclusions: The above results show that two types of antioxidants were identified in the antioxidant extract of E. hyemale exrtracts. Therefore, the potential as a raw material for functional cosmetics has increased.
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KEYWORD
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Equisetum hyemale, Antioxidant, Cosmetics, Hot water extract, HPLC
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